Sequence retrieval

Genome: Methanosarcina acetivorans str. C2A
Start position: 458726
End position: 463162
Length: 4437

DNA sequence
>NC_003552, from 458726 to 463162 (4437 bp); Methanosarcina acetivorans str. C2A TTACATTTTCTTCTTCCGCATGCCCAGGTAGATTCCACCCATTGCTACTACTACAAAGAGAATTCCGACA ATATCGGCCCCTGAAAAAGAGAAATCTCCGTTTTCTGCATTTTCTACGGATTCTTTCTGCATCTCATAAC CCTCGACGTAATTTGAATCTGCTGCCTGTCGGACATCCGGAGCGATCTCCGGAGAATCAACACCGTACCC CGCATCTGATGTCTGCGAGGTCTGGTTGCTGGCCGATTCCCCGGAGTTTGTTTCTGCGGTCTGGTTGCTG GACATACTTTCTTTTGAAACTACGGTAGCTTCCCCTGTACCAGAACCGCCACTGCTGCTACTGCTGCTTT CACTCGGCTCCTGAGAAGTCTCGCTTGATGTTGCCTGTTCAATAGCCTCGGTGAAACCGGGGACTGAGAC CAGACCTGCAACATAGCTGTTTAAGGTCGGGTTTCCGCAGGTATGGTGACAGCAGGCAACTCCGTTTTCT ACAACGGATTCTGCGTACTCCGTAGCAAGGTTCTTGAGGGCCTCATCCGAAGCATCCCAGTTGCCTTCCA GGGCAGTTTCCAGCATTCTGGCCGTGATGGACTGGTATGCATAGGCATTGGTAGCTTTGAGCTCGGCATT GAGTTCGGAATCGGCGACATAGGTTTCATACACCTTGTTCCAGATATCGTCACTTACCAGGTCAGGGTTT GTCTTTTCCCAGCCCCAGAGGTTTTCAACATAGTCCGCTAACTGGCTCACCCCTTCAAAACCGCTTCCCA GCATTCCTTCAATGGATTTGGGGTTCAGGACACGGGTGATAATTTCCCTTGTAAGATACTCATTCAGGGT TTCTATCTCTTCATCCCCCGAATTTTGCAGGTTCATTATGTACGAATCGGGGTACTCGCCTGAAACTGAG GAAACTGCAAGGTTAAGGCCGCCAAGATACTCAAAGGCATCACTATTATCCAGGATTCCGTAGGTACTTG AACTGCGGCTGAAAACCGTAGCCTCAACATCTCCAAGGTTATCCTTAAAAATGTCCGTATTCTCAAGGGA ACTGCCATCTACCTGGAACTGCGAGACAATATCGGCTATACTCTCTCCCCATACATCTTCTCCATAAACA TAGCTCATCCTGTCTATGTAAAGTTCAGCAATTTCACTGGTGCTGGAGTTGCTCGCAGAAACAACATTTG ACAATCCGGTCCCGTAAGTGCCGTCCTCGGGTCCGAAAACCCTCAGAAGAGCCACGGTCATTGAAGCGGT CTCGTTTTGGAGAGATTCGTTAAGGACAAGGAAAAGGTCATTGGTATTCTCCCGGACATAATTCGTATTT TCTTCCGGGTCATAGGAGATATACTCCGGGGTCGGTCTTTCCTCATCTTTTCCGTACTGGTTGTCAGGGT CGTTGTAAGCAAGGTAGACTGCTTTATCGATCAGCTCTACCTTATGGGGGAAAGTGTCCCTGTAGAGCCC CGAAATCTGTACAAGCACGTCAATGCGCGGCCTGCCCAGTTCCGAAGAATTGATCAGTTCGACATCTATG ACCTCATCTCCGTCGTCGTCCCAGACCGGCTTTACTCCCAGGAGGTAGAAGATTTCAGCCTCGGTTACTC CCTCGTTCCGGGTAGATTCCCCAGCCCAGAGGATCAGGCCTATTTTCTTCGGATACTGCCCTTCGTGATT AGCCTGGTAGGTTTCGATTGTCTGGTTAGCCATTTCCTTACCAAGTTCCCACGCCTGCTTCGTGGGGGAC AGTTGTTCGTCAAAGGCGTAGAAGTTCCTCCCCGAGGGAAGCGCGTCCGGCCGGAGTATGGGATCTCCAC CAATATTCGGTTCGATGTATTCACCGTCCAGAGCCTTAAGAACCTGCTGGATTTCATTTTTACTTTCTCC GAGTTTTTCTGCGTATTCCTGGGCCGTGGTCAGGTAACCGGTCACTTCTTCGGAACTGCTCCCGAGAACC TGTATCTGGGCGTCTTCAATACTCACGTTCTGCAGGAGGACAAGGTCAAGAAGGTCAAGAGAAGCGTTTT CAGAACTATTGTATTCAGCCACCTCTTCGGAAAAATCGTCTCCCAGCATCGAGTAGACCATCTGTGTGAG GTTTTCTCCCTCAGGAGAAGTTCCGAGTATGTGAAGCCCATAGGGCATGGAAGTAGTCTTAAGTTCCTCC AGAATATCGTCAAGCTCGTCAAGGAACTCTTCTTCCGTATCCTCACTTTCGGACAGGCTTACGTTCACAA ATTCGTCAAGGCTAAGTTCCATGGTGAGATTAACGATTTCCTGCATACGAAGTTCTTTCAATTCGGATTC GCTGGGAAGGGTCTGGTACTGGGAAATCTTGCTGCTCAACTCGGTGTAATTCCCATAACTTTCGGAAAGC ACAACCGGCGGGACCAGATGGTCTATAATCACCGCATTGCCCCTGCGCTTTGCCTGCATTCCTTCTCCCA TCCCGTCCATGATATAGGGATAAATTACCGGTATGTCCCCGCTAATGATTGCAGGCCACTCGTCACTGAA GAGGCAGAATTCTTTTCCGGGAAGCCATTCCACAGTCCCGTGGCGGCCCATATTCACAATCGCATCGGCT CCGTACTCATTCTGGAGCCAGAGGTAGAATGCGAGGTACTGGTGGTGGGGAGGCAGGTCTCCATCATGGT AGAGAAGTTCGTTGTCCGAAAGCCACCCTCTTGTTGGCTGGGGGGCAAGGATTACATTATCACTGACTTC AACCTTCGGAATTACCAGGAATTTGTCCCCGGAATCGTCTTCATAGACCATTATTTCTCCGGGTGCGGGC CCCCAGGTCTCGAGAACTTCCTGCTGCCTCTCTTCGGGGAGCGCCGAAAACCATTCAAGGTATGTTGATT CTGGAATAAGCTCAACTTCTCCTGACTCGACAAGCCTTTCAAGCTCTCCCGGAGCCCAGGTCCCTATATT CGTACCCTGTTTTAACATCAGGTTTGTTAATTCGCTTTCATTCGGAATAGAGTCCTTATCAATATCATAG CCCTCATCGGCCATTCCTTCAAGGAGATTGTTAATACTCGGGATAACCTCAAGATAAGAAGCTCCTATGC TGTCATTTCCGCAGCCGTGGCTGTAATAGAGGATTGCAACTTTTTTCTCGGATTCGCTCTTTCCTGAAAG GTCGGTCTGGGAAACTGCCCTGTCAACAAGCCATTCCACCTGGGCCTCAACAGGGACATACACTTCGGTA TTTGTTTCGTTATCCAGCTCATTGGAAGCGATCATTATCGGGTCGATCCAACCCCAGGTCTCGGGCCTGT ACAGCCTGATCATATAGGTATTTGTCAGAGGATCGCTAACATTCTGCCATTCGCTGAGGTTCATGTAACC GTTGAGGACGCAGTTGATAACCGGAACTCCCAGTTCTTCAACATCAAAATACTGACTGCGATAGGTGGTC GAGACTATCAGGTCGGCTTTTGTAGAGCTGTTATAATTGAGGAATTTGTCAACATATTCCTTGCTGGACC CGTAGCATGCTATGACATTCACACCCCGGGACTCAAACTCCTCTATGAGCGCATCGATAGGCTGCATGTC CGCAGGATAATAAGAGAGGTAGAACAGTATCCCTACCGTGGGAGCGTTTTCATCAAAAGAATGTTCGCTT TCATTCCTGCTTCCATACCATTCAAAGTATTCACTCGCATTGGCAGTAAAGTAAGAACTGTTAACCATAC CCGGGTGATAAATGGCGCTGGCGGCTCCTTCCGGGGCTGAGACCTGAAGGTCGTCCCGGGCATAGAACAC CTGAGCCAGATAGAAGATCAGGTTATCAAAGTTTGCGTCATTCGTAGCCCCGTACACCCAGTAGTCCTGA AGATAGGCCTTAAGTTCCCCCTCATCCGAAAACGAAGAAGGAAGGGAAGAGTTGTCTATGCTTTCCGGCA GATAAGTGTTATAGCCTATGACAACGGCTCCGTTTGCGATCGCTTCATTGACAGTATTTTCAAGTTTACT GGCACTGTCGGTAAACATATTGATATAAATTATATCCATATCACTGAGATCAACATCCTCTCCACTGGTG GCAGGCAGGTAATAGGAAACACTCATATTAAGGGAAGAGTTGCCGTTAATCCTCTCTGCGAGGGCATTCA ATTTAAGGTCATAATTTGAGTACCCCGTGATTATAGCTACATTGAGGTGGGAAGTGCTACCTGAATTTGA AGAATCCGAATCCTGATTCGAAGAGCCGGATGTTTTTTTCAGGAGTACCAGGTCCGTTCCGGCCTTGATA TCCCCGGACTTGCTGAGAGTCAGGCCGGAAATGGTAGTTCTGTCAAGATAACCGAGGATCTCTTCATGGT CGATATTCTCATTACTTCTCATGGCAAAAGTGAGATTTACAGGCTGACCGTCCTCAATGCTGAATTCGCT TTCATTGGTAAGCCACATCCCTCCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 458726-463162	Sequence	MA0385 cobN cobaltochelatase subunit CobN (Protein is coded in complementary strand; from 463162 to 458726)