DNA sequence >NC_003552, from 3259447 to 3259542 (96 bp); Methanosarcina acetivorans str. C2A GAAAACTGTTGTTAAACGAAGCTGTTGTTAAACGAAGCTGTTGTTAAACGAAGCTGTTGTTAAACAAAGC TGTTGTTAAACGAAGCTGTTGTTAAA Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI