Sequence retrieval

Genome: Escherichia coli O157:H7 str. Sakai
Start position: 3491786
End position: 3491929
Length: 144

DNA sequence
>NC_002695, from 3491786 to 3491929 (144 bp); Escherichia coli O157:H7 str. Sakai TTATTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTC TGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGC TCTG
Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF. 3491695-3492963	Sequence	ECs3507 - hypothetical protein (Protein is coded in complementary strand; from 3492963 to 3491695)