In silico PCR amplification

PCR may be simulated against up-to-date sequenced prokaryotic genomes. This service allows a maximum of 2 mismatches between primers and template, so the stringency of in silico PCR must be consider high.

Experiments against user's sequences may be simulated, and downloadable PHP script is available at

NEW: simulation of PCR againts over 6,000 draft genomes here.


Citing this site

PCR against viral sequences