This approach has not yet been tried in the lab.
We will explain the theoretical experiment performed when filling the
form as shown
In this experiment we will use only one endonuclease (Bsa
recognition sequence C'CNNG_G) to perform an AFLP-PCR-like
experiment. The maximum length of
has been set up to 3000 bp.
JI cuts E. coli
K12 genome 11483 times
(more info here
11483 fragments will be yielded.
By using the experimental conditions introduced in the form, in the
first step, two kinds
of fragments will be computed:
In a second step, as sequence CCNNGG has been introduced in the form as
"Exclusion sequence", all fragments including within their
sequence the "Exclusion sequence" will be removed from final