5'- RE 1 AatII Acc65I AccIII AclI AflII AgeI AhlI Alw44I ApaI ApaLI AscI AseI AsiAI AsiSI Asp718I AspA2I AspLEI AsuII AsuNHI AvrII BamHI BanIII BbeI BbuI BclI BcuI BfaI BfrI BfuCI BglII BlnI BmtI Bpu14I Bsa29I BseAI BseCI BsePI BseX3I BshTI BsiSI BsiWI Bsp106I Bsp119I Bsp120I Bsp13I Bsp1407I Bsp143I Bsp19I BspAUI BspCI BspDI BspEI BspHI BspLU11I BspMAI BspT104I BspTI BspXI BsrGI BssHI BssHII Bst98I BstAUI BstBI BstENII BstHHI BstKTI BstZI Bsu15I BsuTUI CciNI CfoI Cfr42I Cfr9I ClaI Csp45I Csp6I CspAI DpnII EagI EclXI Eco52I EcoRI EcoT22I FatI FauNDI FbaI FseI FunII HapII HhaI Hin6I HinP1I HindIII HpaII HpyCH4IV Hsp92II HspAI KasI Kpn2I KpnI Ksp22I KspI Kzo9I MaeI MaeII MboI MfeI MluI Mly113I Mph1103I MreI MroI MroNI MseI MspCI MspI MunI MvrI NarI NcoI NdeI NdeII NgoAIV NgoMIV NheI NlaIII NotI NsiI NspV PacI PaeI PaeR7I PagI PauI PciI Pfl23II PinAI Ple19I PshBI Psp124BI Psp1406I PspAI PspLI PspOMI PstI PvuI RcaI SacI SacII SalI Sau3AI SbfI SdaI Sfr274I Sfr303I SfuI SgfI SgrBI SlaI SpaHI SpeI SphI Sse8387I Sse9I SspBI SstI SstII StrI SunI TaiI TaqI TasI TliI Tru1I Tru9I TscI Tsp509I TspEI TthHB8I Vha464I VneI VspI XbaI XhoI XmaCI XmaI XmaIII XmaJI XspI ZhoI Zsp2I 1 1 RE 2 AatII Acc65I AccIII AclI AflII AgeI AhlI Alw44I ApaI ApaLI AscI AseI AsiAI AsiSI Asp718I AspA2I AspLEI AsuII AsuNHI AvrII BamHI BanIII BbeI BbuI BclI BcuI BfaI BfrI BfuCI BglII BlnI BmtI Bpu14I Bsa29I BseAI BseCI BsePI BseX3I BshTI BsiSI BsiWI Bsp106I Bsp119I Bsp120I Bsp13I Bsp1407I Bsp143I Bsp19I BspAUI BspCI BspDI BspEI BspHI BspLU11I BspMAI BspT104I BspTI BspXI BsrGI BssHI BssHII Bst98I BstAUI BstBI BstENII BstHHI BstKTI BstZI Bsu15I BsuTUI CciNI CfoI Cfr42I Cfr9I ClaI Csp45I Csp6I CspAI DpnII EagI EclXI Eco52I EcoRI EcoT22I FatI FauNDI FbaI FseI FunII HapII HhaI Hin6I HinP1I HindIII HpaII HpyCH4IV Hsp92II HspAI KasI Kpn2I KpnI Ksp22I KspI Kzo9I MaeI MaeII MboI MfeI MluI Mly113I Mph1103I MroI MroNI MseI MspCI MspI MunI MvrI NarI NcoI NdeI NdeII NgoAIV NgoMIV NheI NlaIII NotI NsiI NspV PacI PaeI PaeR7I PagI PauI PciI Pfl23II PinAI Ple19I PshBI Psp124BI Psp1406I PspAI PspLI PspOMI PstI PvuI RcaI SacI SacII SalI Sau3AI SbfI SdaI Sfr274I Sfr303I SfuI SgfI SgrBI SlaI SpaHI SpeI SphI Sse8387I Sse9I SspBI SstI SstII StrI SunI TaiI TaqI TasI TliI Tru1I Tru9I TscI Tsp509I TspEI TthHB8I Vha464I VneI VspI XbaI XhoI XmaCI XmaI XmaIII XmaJI XspI ZhoI Zsp2I -3'
Migroorganism
1 A,T,G,C and N are allowed.
Note: Selective nucleotide on the right are the ones in the upper strand of the sequence.